Nucleotide Sequence of Beet Necrotic Yellow Vein Virus RNA-1

The complete nucleotide sequence of beet necrotic yellow vein virus RNA-1 is presented. The R N A molecule is 6746 nucleotides long excluding the poly(A) tail and has one long open reading f lame encoding a polypeptide of M r 237 389. The 3' terminal 60 residues of BNYVV RNA-1 display extensive sequence homology with the corresponding portions of BNYVV RNA-2, -3 and -4. Addi t ional 3' terminal homology exists between RNA-1 and -2. The sequence of the M r 237389 RNAl encoded polypeptide shares domains of amino acid homology with polypeptides thought to be involved in replication of R N A from tobacco mosaic virus and several other viruses. Amino acid sequence homologies between two open reading frames of BNYVV R N A 2 and two frames of RNA-f l from barley stripe mosaic virus have also been detected.